SEMA4D/CD100 antibody

Catalog No. ABIN6939082

The Mouse Monoclonal anti-SEMA4D/CD100 antibody (Clone A8) (ABIN6939082) specifically detects SEMA4D/CD100 in FACS, IF and Func. The antibody is reactive with Human, Mouse and Monkey samples.

Quick Overview for SEMA4D/CD100 antibody (ABIN6939082)

Target: SEMA4D/CD100 (SEMA4D) (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D))

Reactivity: Human, Mouse, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This SEMA4D/CD100 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)

Clone: A8

Product Details anti-SEMA4D/CD100 Antibody

Purpose: CD100 (Semaphorin-4D) Antibody

Specificity: Recognizes a homodimeric protein comprised of 50 kDa subunits, identified as CD100. It is expressed on majority of haemopoietic cells (B, T, NK and myeloid cells) and is absent from bone marrow, erythrocytes, eosinophils and endothelial cells. Its expression is increased after PHA-activation. CD100 was shown to associate with different partner molecules in T cells such as CD45, a key molecule with protein tyrosine phosphatase activity involved in T-cell transduction, and a Serine kinase. It plays a role in homotypic cell adhesion and in T cell activation.

Cross-Reactivity (Details): Human, Monkey, Mouse,

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: PHA stimulated human peripheral blood lymphocytes

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: Daudi, Raji, HUT-78, Kg1a, U937 and human lymphocytes. Human tonsils and lymph nodes.

Known Application: Functional Studies (Order Ab without Azide),Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for SEMA4D/CD100

Target: SEMA4D/CD100 (SEMA4D) (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D))

Alternative Name: SEMA4D

Background:

COLL4, Collapsin4, GR3, M sema G, MSEMA, SEMA4D, Semacl2, SemaH, SEMAJ, Semaphorin C like 2, Semaphorin H, Semaphorin J, Semaphorin-4D, Semcl2

Daudi, HUT-78, Kg1a, Raji, U937 and human lymphocytes. Human tonsils and lymph nodes.

Molecular Weight: 50kDa (Monomer)

Gene ID: 10507, 494406

UniProt: Q92854

Pathways: Regulation of Cell Size