UPK1A antibody (AA 114-173)

Catalog No. ABIN6939114

The Mouse Monoclonal anti-UPK1A antibody has been validated for ELISA, Coat and IHC (f). It is suitable to detect UPK1A in samples from Human.

Quick Overview for UPK1A antibody (AA 114-173) (ABIN6939114)

Target: UPK1A (Uroplakin 1a (UPK1A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This UPK1A antibody is un-conjugated

Application: ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: UPK1A-2921

Product Details anti-UPK1A Antibody

Binding Specificity: AA 114-173

Specificity: The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is found in the asymmetrical unit membrane (AUM) where it can complex with other transmembrane 4 superfamily proteins. It may play a role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions. The protein may also play a role in tumor suppression.

Cross-Reactivity (Details): Human,

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa 114-173) of human Uroplakin 1A (UPK1A) protein (exact sequence is proprietary)

Isotype: IgG2b

Application Details

Application Notes:

Positive Control: Urinary Bladder.

Known Application: ELISA (For coating, order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Expiry Date: 24 months

Target Details for UPK1A

Target: UPK1A (Uroplakin 1a (UPK1A))

Alternative Name: UPK1A

Background: UP1A, UPIA, UPKA, TSPAN21,Uroplakin 1A (Urothelial Differentiation Marker)
Cellular localisation: Cell Surface

Molecular Weight: 29kDa

Gene ID: 11045, 159309

UniProt: O00322