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Creatine Kinase, Brain (CKB) antibody Primary Antibody

CKB Reactivity: Human Coat, ELISA, FACS, IF, IHC (fro) Host: Mouse Monoclonal 2ba6
Catalog No. ABIN6939143
$494.31
Plus shipping costs $45.00
100 μg ABIN6939143
100 μg ABIN6939143
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  • Target
    Reactivity
    Human
    Host
    Mouse
    Clonality
    Monoclonal
    Conjugate
    Un-conjugated
    Application
    Coating (Coat), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificity
    The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. The specificity of this monoclonal antibody to its intended target was validated by HuProt Array containing more than 19,000 full-length, correctly-folded human proteins. Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This MAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB. It shows minimal reactivity with other human serum proteins.
    Cross-Reactivity (Details)
    Predicted to react with Chimpanzee, Rhesus Monkey, Dog, Cow, Mouse, Rat, Chicken, Zebrafish, Frog.
    Purification
    Purified by Protein A/G
    Immunogen
    Human CKBB protein
    Clone
    2ba6
    Isotype
    IgG1, kappa
  • Application Notes

    Positive Control: Cerebellum.

    Known Application: ELISA (For coating, order Ab without BSA),Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Frozen Only) (0.5-1.0 μg/mL for 30 minutes at RT) Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Expiry Date
    24 months
  • Target
    Alternative Name
    CKB (CKB Antibody Abstract)
    Synonyms
    B-CK, CKBB, b-ck, ckbb, CKB, Bck, Ck-3, Ck3, Ckbb, bck, ckb, cb594, wu:fj36f10, wu:fj37d11, Ckbr, creatine kinase B, creatine kinase, brain L homeolog, creatine kinase B-type, creatine kinase, brain, creatine kinase, brain b, CKB, ckb.L, Ckb, ckb, ckbb
    Molecular Weight
    43kDa (Monomer), 86kDa (Dimer)
    Gene ID
    1152
    UniProt
    P12277
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