GM-CSF antibody

Catalog No. ABIN6939189

The Rat Monoclonal anti-GM-CSF antibody (Clone BVD2-21C11) (ABIN6939189) specifically detects GM-CSF in WB, IHC, FACS and IF. The antibody is reactive with Human, Cynomolgus and Rhesus Monkey samples.

Quick Overview for GM-CSF antibody (ABIN6939189)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Reactivity: Human, Cynomolgus, Rhesus Monkey

Host: Rat

Clonality: Monoclonal

Conjugate: This GM-CSF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: BVD2-21C11

Product Details anti-GM-CSF Antibody

Purpose: GM-CSF (Granulocyte/Macrophage - Colony Stimulating Factor) Antibody

Specificity: Granulocyte/macrophage - Colony-stimulating factor (GM-CSF) is a hematopoietic factor that is produced by activated T-cells, B-cells, mast cells, macrophages, fibroblasts, and endothelial cells. In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human GM-CSF protein

Isotype: IgG2a, kappa

Application Details

Application Notes:

Positive Control: Lymph node or tonsil.

Known Application: Neutralization Studies (Order Ab without Azide), Immunoprecipitation (0.5-1 μg/500 μg protein lysate), Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Frozen) (0.5-1 μg/mL for 30 minutes at RT)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for GM-CSF

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Alternative Name: CSF2

Background:

Burst Promoting Activity, Eosinophil Colony Stimulating Factor, Molgramostin, Pluripoietin Alpha, Sargramostim

Lymph node or tonsil.

Molecular Weight: 22kDa

Gene ID: 1437, 1349

UniProt: P04141

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin