Cathepsin D antibody (AA 104-250)

Catalog No. ABIN6939215

The Mouse Monoclonal anti-Cathepsin D antibody has been validated for IHC and StM. It is suitable to detect Cathepsin D in samples from Human.

Quick Overview for Cathepsin D antibody (AA 104-250) (ABIN6939215)

Target: Cathepsin D (CTSD)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cathepsin D antibody is un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Clone: CTSD-3082

Product Details anti-Cathepsin D Antibody

Binding Specificity: AA 104-250

Purification: Purified by Protein A/G

Immunogen: Recombinant fragment of human Cathepsin D protein (around aa 104-250) (exact sequence is proprietary)

Isotype: IgG2b kappa

Application Details

Application Notes:

Positive Control: K-562, A431 or SK-BR3 cells. Kidney, Liver, Lung or Breast.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Cathepsin D

Target: Cathepsin D (CTSD)

Alternative Name: CTSD

Background: Cathepsin D is a ubiquitously expressed lysosomal aspartyl protease involved in the normal degradation of proteins. It is synthesized as an inactive 43 kDa preprocathepsin D that is cleaved and glycosylated to form a 46 kDa procathepsin D and then further cleaved to produce 28 kDa and 15 kDa subunits (heavy and light chains, respectively). Cathepsin D exhibits pepsin-like activity and plays a role in protein turnover and in the proteolytic activation of hormones and growth factors. Mutations in this gene play a causal role in neuronal ceroid lipofuscinosis-10 and may be involved in the pathogenesis of several other diseases, including breast cancer and possibly Alzheimer's disease.

Molecular Weight: 46kDa (Procathepsin D), 28kDa (Cathepsin D)

Gene ID: 1509

UniProt: P07339

Pathways: Peptide Hormone Metabolism