EGFR antibody (Extracellular Domain)
Quick Overview for EGFR antibody (Extracellular Domain) (ABIN6939288)
Target
See all EGFR AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
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Binding Specificity
- Extracellular Domain
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Specificity
- This MAb recognizes a protein of 170 kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.
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No Cross-Reactivity
- Mouse (Murine)
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Purification
- Purified by Protein A/G
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Immunogen
- Recombinant extracellular domain of human EGFR protein
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Isotype
- IgG2a kappa
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anti-Epidermal Growth Factor Receptor (EGFR) (AA 693-893) antibody
EGFR Reactivity: Human WB, IHC, ELISA, FACS Host: Mouse Monoclonal 7A6F12 unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (AA 1163-1191), (C-Term) antibodyEGFR Reactivity: Human WB, IHC (p) Host: Mouse Monoclonal 688CT33-1-3 unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (AA 888-1210) antibodyEGFR Reactivity: Human WB, IHC Host: Mouse Monoclonal C4 unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (C-Term) antibodyEGFR Reactivity: Human, Mouse, Rat, Monkey, Pig, Cow, Chicken, Dog, Horse, Rabbit, Guinea Pig, Avian, Cat, Donkey, Goat, Hamster, Sheep WB, IHC (p), IHC (fro) Host: Goat Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (pSer1071) antibodyEGFR Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (pSer1026) antibodyEGFR Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (pSer695) antibodyEGFR Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (AA 693-893) antibodyEGFR Reactivity: Human WB, ELISA, FACS Host: Mouse Monoclonal 5E10D3 unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) antibodyKD Validated EGFR Reactivity: Human WB, FACS, IF (p) Host: Rabbit Monoclonal 24GB4395 unconjugated Recombinant Antibody
anti-Epidermal Growth Factor Receptor (EGFR) (C-Term) antibodyEGFR Reactivity: Human, Rat WB, IHC, ELISA, IP Host: Rabbit Polyclonal unconjugated
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Application Notes
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Positive Control: A431 cells, Breast or Bladder cancer.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.
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Restrictions
- For Research Use only
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Concentration
- 200 μg/mL
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Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C,-80 °C
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Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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Expiry Date
- 24 months
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- EGFR (Epidermal Growth Factor Receptor (EGFR))
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Alternative Name
- EGFR
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Molecular Weight
- ~170kDa (wild type) and ~145kDa (vIII variant)
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Gene ID
- 1956
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UniProt
- P00533
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Pathways
- NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins
Target
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