AMACR antibody

Catalog No. ABIN6939430

The Mouse Monoclonal anti-AMACR antibody has been validated for WB, IHC and StM. It is suitable to detect AMACR in samples from Human.

Quick Overview for AMACR antibody (ABIN6939430)

Target: AMACR (alpha-Methylacyl-CoA Racemase (AMACR))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This AMACR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)

Clone: AMACR-1723

Product Details anti-AMACR Antibody

Specificity: This antibody recognizes a protein of 42 kDa, which is identified as AMACR, also known as p504S. It is an enzyme that is involved in bile acid biosynthesis and -oxidation of branched-chain fatty acids. AMACR is essential in lipid metabolism. It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands. AMACR is expressed in normal liver (hepatocytes), kidney (tubular epithelial cells) and gall bladder (epithelial cells). Expression has also been found in lung (bronchial epithelial cells) and colon (colonic surface epithelium). AMACR expression can also be found in hepatocellular carcinoma and kidney carcinoma. Past studies have also shown that AMACR is expressed in various colon carcinomas (well, moderately and poorly differentiated) and over expressed in prostate carcinoma.

Purification: Purified by Protein A/G

Immunogen: Recombinant full-length human AMACR protein

Isotype: IgG2b kappa

Application Details

Application Notes:

Positive Control: PC3, HEK cells. Prostate Adenocarcinoma, Human Liver Tissue.

Known Application: Western Blot (0.5-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for AMACR

Target: AMACR (alpha-Methylacyl-CoA Racemase (AMACR))

Alternative Name: AMACR

Molecular Weight: 42kDa

Gene ID: 23600

UniProt: Q9UHK6

Pathways: Monocarboxylic Acid Catabolic Process