Recombinant CD15 antibody

Catalog No. ABIN6939478

The Rabbit Monoclonal anti-CD15 antibody (Clone FUT4-1478R) (ABIN6939478) specifically detects CD15 in FACS, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Recombinant CD15 antibody (ABIN6939478)

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD15 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: FUT4-1478R

Product Details anti-CD15 Antibody

Purpose: Recombinant CD15 / FUT4 (Reed-Sternberg Cell Marker) Antibody

Specificity: CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.

Purification: 200ug/ml of Ab Purified by Protein A Column.

Immunogen: Recombinant human full-length CD15 protein

Isotype: IgG, kappa

Application Details

Application Notes:

Positive Control: U937 cells, Reed-Sternberg's cells in Hodgkin's lymphoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD15

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Alternative Name: FUT4

Background:

3 Fucosyl N Acetyl Lactosamine; Alpha (1,3) Fucosyltransferase; Alpha 13 fucosyltransferase FucT; ELAM Ligand Fucosyltransferase; ELFT; FCT3A; Fuc-TIV; Fucosyltransferase 4 Alpha 1 3 Fucosyltransferase Myeloid Specific; Fucosyltransferase 4; Galactoside 3 L Fucosyltransferase; Lewis X; LeX; SSEA1; Stage Specific Embryonic Antigen 1

Reed-Sternberg's cells in Hodgkin's lymphoma., U937 cells

Molecular Weight: 220kDa

Gene ID: 2526, 654379

UniProt: P22083