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Glypican 3 antibody

GPC3 Reactivity: Human, Rat FACS, IF, IHC, StM Host: Rabbit Monoclonal GPC3-1534R unconjugated
Catalog No. ABIN6939528
$575.85
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  • Target See all Glypican 3 (GPC3) Antibodies
    Glypican 3 (GPC3)
    Reactivity
    • 182
    • 60
    • 51
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Rat
    Host
    • 113
    • 69
    • 2
    Rabbit
    Clonality
    • 95
    • 89
    Monoclonal
    Conjugate
    • 63
    • 14
    • 9
    • 8
    • 8
    • 7
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This Glypican 3 antibody is un-conjugated
    Application
    • 106
    • 100
    • 62
    • 59
    • 50
    • 38
    • 17
    • 17
    • 5
    • 2
    • 2
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)
    Immunogen
    Recombinant human full-length GPC3 protein
    Clone
    GPC3-1534R
    Isotype
    IgG
  • Application Notes

    Positive Control: HepG2, 293T cells. Hepatocellular carcinoma.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA buffer, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Expiry Date
    24 months
  • Target
    Glypican 3 (GPC3)
    Alternative Name
    GPC3 (GPC3 Products)
    Synonyms
    GPC3 antibody, sgb antibody, dgsx antibody, sdys antibody, sgbs antibody, oci-5 antibody, sgbs1 antibody, DGSX antibody, GTR2-2 antibody, MXR7 antibody, OCI-5 antibody, SDYS antibody, SGB antibody, SGBS antibody, SGBS1 antibody, Glypican-3 antibody, glypican 3 antibody, gpc3 antibody, GPC3 antibody, Gpc3 antibody
    Background
    Glypican-3 (GPC3) is a glycosylphospatidyl inositol-anchored membrane protein, which may also be found in a secreted form. Anti-GPC3 has been identified as a useful tumor marker for the diagnosis of hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell tumors, and Wilm's tumor. In patients with HCC, GPC3 is overexpressed in neoplastic liver tissue and elevated in serum, but is undetectable in normal liver, benign liver, and the serum of healthy donors. GPC3 expression is also found to be higher in HCC liver tissue than in cirrhotic liver or liver with focal lesions such as dysplastic nodules and areas of hepatic adenoma (HA) with malignant transformation. In the context of testicular germ cell tumors, GPC3 expression is up regulated in certain histologic subtypes, specifically yolk sac tumors and choriocarcinoma. A high level of GPC3 expression is also found in some types of embryonal tumors, such as Wilm's tumor and hepatoblastoma, with a low or undetectable expression in normal adjacent tissue. In patients with thyroid cancer, expression of GPC3 is dramatically enhanced in certain types of cancers: 100 % in follicular carcinoma and 70 % in papillary carcinoma. Expression of GPC3 in follicular carcinoma is significantly higher than that of follicular adenoma. In contrast, GPC3 is not expressed in anaplastic carcinoma.
    Molecular Weight
    67kDa
    Gene ID
    2719
    UniProt
    P51654
    Pathways
    Glycosaminoglycan Metabolic Process
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