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Recombinant HLA DQ antibody

HLA-DQ Reactivity: Human, Pig FACS, IF, IHC (fro) Host: Mouse Monoclonal rSPV-L3 unconjugated Recombinant Antibody
Catalog No. ABIN6939633
  • Target See all HLA DQ (HLA-DQ) products
    HLA DQ (HLA-DQ) (MHC Class II HLA-DQ (HLA-DQ))
    Antibody Type
    Recombinant Antibody
    Reactivity
    • 28
    • 14
    • 2
    Human, Pig
    Host
    • 26
    • 4
    Mouse
    Clonality
    • 30
    Monoclonal
    Conjugate
    • 17
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This HLA DQ antibody is un-conjugated
    Application
    • 29
    • 22
    • 7
    • 4
    • 4
    • 3
    • 2
    • 2
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificity
    Recognizes a DQ antigen, which is a dimer of 60 kDa. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons, exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation.This MAb strongly blocks cytotoxicity activity of T4-positive cytotoxic T cell clones.
    Purification
    Purified by Protein A/G
    Immunogen
    T4-positive CTL clone HG-38
    Clone
    RSPV-L3
    Isotype
    IgG1 kappa
  • Application Notes

    Positive Control: Raji cells. Tonsil or lymph node.

    Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Frozen only) (0.5-1 μg/mL for 30 minutes at RT)Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Expiry Date
    24 months
  • Target
    HLA DQ (HLA-DQ) (MHC Class II HLA-DQ (HLA-DQ))
    Alternative Name
    HLA-DQ (HLA-DQ Products)
    Molecular Weight
    60kDa
    Gene ID
    3117, 3118, 3119
    UniProt
    P01908, P01909, P01920
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