IGHM antibody

Catalog No. ABIN6939733

This Mouse Monoclonal antibody specifically detects IGHM in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for IGHM antibody (ABIN6939733)

Target: IGHM (Immunoglobulin Heavy Constant mu (IGHM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IGHM antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: IGHM-1623

Product Details anti-IGHM Antibody

Purpose: IgM (Immunoglobulin Mu Heavy Chain) (B-Cell Marker) Antibody

Specificity: Recognizes a protein of 75 kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. IgM antibody is prominent in early immune responses to most antigens. Aberrant levels are associated with immune deficiency states, hereditary deficiencies, myeloma, Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Heavy chain of human IgM

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.

Known Application: ELISA (For coating, order antibody without BSA), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for IGHM

Target: IGHM (Immunoglobulin Heavy Constant mu (IGHM))

Alternative Name: IGHM

Background:

AGM1, IGHM, Constant Region of Heavy Chain of IgM, Ig Mu Chain C Region

293T, Raji or hPBL cells. Tonsil or Spleen. Human Serum

Molecular Weight: 50-75kDa

Gene ID: 3507, 510635

UniProt: P01871