C-JUN antibody

Catalog No. ABIN6939837

This Mouse Monoclonal antibody specifically detects C-JUN in WB, IHC, IF, FACS and StM. It exhibits reactivity toward Human and Rat.

Quick Overview for C-JUN antibody (ABIN6939837)

Target: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This C-JUN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Clone: C-J 4C4-1

Product Details anti-C-JUN Antibody

Immunogen: c-Jun protein phosphorylated at T91 and T93

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: HEK293, HeLA or A-431 cells, Human normal lung or bladder tissue.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for C-JUN

Target: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Alternative Name: JUN

Background: This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.

Molecular Weight: 39kDa

Gene ID: 3725

UniProt: P05412

Pathways: MAPK Signaling, RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling, S100 Proteins