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Cytokeratin 18 antibody (Keratin 18) Primary Antibody

KRT18 Reactivity: Human, Mouse, Rat, Cat, Cow, Dog, Ferret, Goat, Hamster, Horse, Marmoset, Pig, Sheep FACS, IF, IHC, StM, WB Host: Rabbit Monoclonal KRT18-2819R unconjugated
Catalog No. ABIN6939939
$537.38
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  • Target See all Cytokeratin 18 (KRT18) Antibodies
    Cytokeratin 18 (KRT18)
    Antibody Type
    Recombinant Antibody
    Reactivity
    • 324
    • 156
    • 124
    • 62
    • 42
    • 42
    • 36
    • 32
    • 29
    • 25
    • 24
    • 21
    • 21
    • 21
    • 9
    • 9
    • 8
    • 8
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Cat, Cow, Dog, Ferret, Goat, Hamster, Horse, Marmoset, Pig, Sheep
    Host
    • 186
    • 157
    • 7
    • 6
    • 2
    • 1
    Rabbit
    Clonality
    • 201
    • 158
    Monoclonal
    Conjugate
    • 165
    • 29
    • 23
    • 10
    • 9
    • 9
    • 9
    • 9
    • 9
    • 9
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    This Cytokeratin 18 antibody is un-conjugated
    Application
    • 224
    • 134
    • 130
    • 108
    • 83
    • 69
    • 58
    • 54
    • 42
    • 40
    • 33
    • 28
    • 11
    • 10
    • 8
    • 7
    • 2
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM), Western Blotting (WB)
    Specificity
    This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
    No Cross-Reactivity
    Chicken, Xenopus laevis
    Purification
    Purified by Protein A/G
    Immunogen
    Recombinant human full-length KRT18 protein
    Clone
    KRT18-2819R
    Isotype
    IgG
  • Application Notes

    Positive Control: HCT116, MCF-7, HeLa cells, Breast Cancer.

    Known Application: Flow Cytometry (0.5-1 μg/million cells),Western Blot (0.5-2 μg/mL),Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Expiry Date
    24 months
  • Target
    Cytokeratin 18 (KRT18)
    Alternative Name
    KRT18 (KRT18 Products)
    Synonyms
    CK18, K18, Krt1-18, CYK18, DreK18, cb83, dapk1, sb:cb83, wu:fa13f02, wu:fb36b04, krt18, MGC64569, MGC75922, KRT18, keratin 18, keratin 18, type I L homeolog, keratin 18, type I, keratin, type I cytoskeletal 18, K18, simple type I keratin, Keratin, type I cytoskeletal 18, Krt18, KRT18, krt18, krt18.L, LOC700569, LOC100136778, k1c18, LOC100008885
    Molecular Weight
    45kDa
    Gene ID
    3875
    UniProt
    P05783
    Pathways
    Apoptosis, Caspase Cascade in Apoptosis
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