MYOD1 antibody (AA 180-189)
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- Target See all MYOD1 Antibodies
- MYOD1 (Myogenic Differentiation 1 (MYOD1))
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Binding Specificity
- AA 180-189
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Reactivity
- Human, Mouse, Rat, Chicken
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This MYOD1 antibody is un-conjugated
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Application
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Staining Methods (StM), Immunostaining (ISt)
- Specificity
- Recognizes a phosphor-protein of 45 kDa, identified as MyoD1. The epitope of this MAb maps between amino acid 180-189 in the C-terminal of Mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm's tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing's sarcomas and alveolar soft part sarcomas.
- Purification
- Purified by Protein A/G
- Immunogen
- Recombinant MyoD of Mouse origin with an epitope mapping to amino acids 180-189.
- Clone
- SPM427
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product MYOD1 Primary Antibody
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- Application Notes
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Positive Control: Rhabdomyosarcoma.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 1 mM EDTA Buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
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- Target
- MYOD1 (Myogenic Differentiation 1 (MYOD1))
- Alternative Name
- MYOD1 (MYOD1 Products)
- Synonyms
- MYF3 antibody, MYOD antibody, PUM antibody, bHLHc1 antibody, MyoD antibody, XMyoD antibody, XtmyoD antibody, mf25 antibody, myf3 antibody, pum antibody, MUF3 antibody, AI503393 antibody, Myod-1 antibody, etID309723.25 antibody, myod antibody, wu:fb57a01 antibody, zgc:136744 antibody, MYOD1 antibody, myod1 antibody, mf1 antibody, myod1-a antibody, myod1-b antibody, myogenic differentiation 1 antibody, MYOD protein antibody, myogenic factor MyoD1 antibody, myogenic differentiation 1 S homeolog antibody, myogenic differentiation 1 L homeolog antibody, MYOD1 antibody, myod1 antibody, Myod1 antibody, myod antibody, myod1.S antibody, myod1.L antibody
- Molecular Weight
- 45kDa
- Gene ID
- 4654
- UniProt
- P15172
- Pathways
- Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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