Recombinant NGFR antibody (AA 281-421)

Catalog No. ABIN6940198

This Rabbit Monoclonal antibody specifically detects NGFR in IHC, Diff and StM. It exhibits reactivity toward Human.

Quick Overview for Recombinant NGFR antibody (AA 281-421) (ABIN6940198)

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This NGFR antibody is un-conjugated

Application: Immunohistochemistry (IHC), Differentiation (Diff), Staining Methods (StM)

Clone: NGFR-2550R

Product Details anti-NGFR Antibody

Binding Specificity: AA 281-421

Specificity: It recognizes a glycoprotein of 75 kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). NGFR is expressed in various neural crest cells and their tumors such as melanocytes, melanomas, neuroblastomas, pheochromocytomas and neurofibromas. Reportedly, anti-NGFR is a reliable marker for desmoplastic and neurotropic melanomas. NGFR is expressed in mature non-neural cells such as perivascular cells, dental pulp cells, lymphoidal follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells, and myoepithelial cells. Anti-NGFR stains the myoepithelial cells of breast ducts and intra-lobular fibroblasts of breast ducts.

Purification: Purified by Protein A/G

Immunogen: Recombinant human p75 NGFR protein fragment (around aa 281-421) (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes:

Positive Control: Neuronal axons, Schwann cells and perineural cells of peripheral nerves or tumors of nerve sheath differentiation e. G. Schwannoma, Neurofibroma. Soma and axons of sensory neurons and ganglionic satellite cells. Melanomas.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for NGFR

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Alternative Name: NGFR

Molecular Weight: 75kDa

Gene ID: 4804

UniProt: P08138

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding