Plakophilin 1 antibody (N-Term)

Catalog No. ABIN6940317

The Mouse Monoclonal anti-Plakophilin 1 antibody has been validated for WB, IF and FACS. It is suitable to detect Plakophilin 1 in samples from Human and Mouse.

Quick Overview for Plakophilin 1 antibody (N-Term) (ABIN6940317)

Target: Plakophilin 1 (PKP1)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Plakophilin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 10B2

Product Details anti-Plakophilin 1 Antibody

Binding Specificity: N-Term

Specificity: Recognizes a protein of 75 kDa, identified as Plakophilin-1. Its epitope maps between aa 1-27 human Plakophilin-1. Plakophilins 1, 2, 3 and 4 (PKP1-4) influence development and participate in linking cadherins to cytoskeletal intermediate filaments. Plakophilins 1-4 contain arm-repeat (armadillo) domains and localize to nuclei and cell desmosomes (cell-cell junctions found in suprabasal layers of stratifying epithelia that undergo mechanical stress). Plakophilin 2 is important for desmosome assembly and is an essential morphogenic factor and architectural component of the heart.

Purification: Purified by Protein A/G

Immunogen: N-terminus of human Plakophilin-1 protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: U87, HeLa and A431 cells.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1.0 μg/mL)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Plakophilin 1

Target: Plakophilin 1 (PKP1)

Alternative Name: PKP1

Molecular Weight: 75kDa

Gene ID: 5317

UniProt: Q13835