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Cytochrome C antibody (AA 1-80)

This Mouse Monoclonal antibody specifically detects Cytochrome C in WB, IHC, FACS, IF and StM. It exhibits reactivity toward Human, Rat, Mouse, Horse, Dog, Pigeon, Drosophila melanogaster and Frog.
Catalog No. ABIN6940319

Quick Overview for Cytochrome C antibody (AA 1-80) (ABIN6940319)

Target

See all Cytochrome C (CYCS) Antibodies
Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Reactivity

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Human, Rat, Mouse, Horse, Dog, Pigeon, Drosophila melanogaster, Frog

Host

  • 113
  • 107
  • 5
  • 1
Mouse

Clonality

  • 138
  • 87
  • 1
Monoclonal

Conjugate

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  • 7
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  • 2
  • 1
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This Cytochrome C antibody is un-conjugated

Application

  • 179
  • 91
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  • 64
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  • 30
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  • 14
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Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Staining Methods (StM)

Clone

7H8-2C12
  • Binding Specificity

    • 43
    • 16
    • 11
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 2
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    • 2
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    • 1
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    • 1
    • 1
    • 1
    AA 1-80

    Specificity

    It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).

    Purification

    Purified by Protein A/G

    Immunogen

    Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon cytochrome c

    Isotype

    IgG2b kappa
  • Application Notes

    Positive Control: K-562, HL-60, Jurkat, NIH3T3 or PC-3 cells. Liver or Cardiac muscle.

    Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    Restrictions

    For Research Use only
  • Concentration

    200 μg/mL

    Buffer

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-80 °C

    Storage Comment

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    Expiry Date

    24 months
  • Target

    Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

    Alternative Name

    CYCS

    Molecular Weight

    15kDa

    Gene ID

    54205

    UniProt

    P99999

    Pathways

    Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
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