PPP3R1 antibody

Catalog No. ABIN6940383

The Mouse Monoclonal anti-PPP3R1 antibody (Clone BE2-1) (ABIN6940383) specifically detects PPP3R1 in WB, ELISA, IHC, StM and Coat. The antibody is reactive with Human and Xenopus laevis samples.

Quick Overview for PPP3R1 antibody (ABIN6940383)

Target: PPP3R1 (Protein Phosphatase 3, Regulatory Subunit B, alpha (PPP3R1))

Reactivity: Human, Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This PPP3R1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Staining Methods (StM), Coating (Coat)

Clone: BE2-1

Product Details anti-PPP3R1 Antibody

Purification: Purified by Protein A/G

Immunogen: Recombinant full-length human Calcineurin B protein

Isotype: IgG2a kappa

Application Details

Application Notes:

Positive Control: HeLa, MCF-7 or A431 cells. Human brain, breast or stomach tissue.

Known Application: ELISA (For coating, order antibody without BSA), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for PPP3R1

Target: PPP3R1 (Protein Phosphatase 3, Regulatory Subunit B, alpha (PPP3R1))

Alternative Name: PPP3R1

Background: Calcineurin is an enzyme that dephosphorylates serine and threonine residues in proteins. It is a heterodimer of a 59 kDa catalytic A subunit and a 19 kDa regulatory B subunit that is activated by the binding of calcium ions and calmodulin. Calcineurin is expressed in many tissues, but its levels are highest in the brain, where it may play a role in learning and memory. It has many substrates, including NFAT, a transcription factor that is activated by dephosphorylation. Complexes of the immuno-suppressants cyclosporine and FK506 with immunophilin proteins such as cyclophilin and FKBP12 are potent and specific inhibitors of Calcineurin activity. Alterations in Calcineurin activity are suspected to play a role in cardiac hypertrophy and graft versus host disease in organ transplantation.

Molecular Weight: 19kDa

Gene ID: 5534

UniProt: P63098

Pathways: RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Protein targeting to Nucleus, VEGF Signaling, BCR Signaling