PTGS2 antibody (AA 442-572)

Catalog No. ABIN6940431

This Mouse Monoclonal antibody specifically detects PTGS2 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for PTGS2 antibody (AA 442-572) (ABIN6940431)

Target: PTGS2 (Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PTGS2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: COX2-1941

Product Details anti-PTGS2 Antibody

Binding Specificity: AA 442-572

Purpose: Cycloxygenase-2 (COX-2) Antibody

Specificity: Prostaglandins are a diverse group of autocrine and paracrine hormones that mediate many cellular and physiologic processes. Prostaglandin H2 (PGH2) is an intermediate molecule in formation of the prostaglandins. Cyclooxygenase-1 (Cox-1) and cyclooxygenase-2 (Cox-2) are prostaglandin synthases that catalyze the formation of PGH2 from arachidonic acid (AA). Cox-1 and Cox-2 are isozymes of prostaglandin-endoperoxidase synthase (PTGS). Cox-1 is constitutively expressed in most tissues and is thought to serve in general housekeeping functions. Cox-2 is efficiently induced in migratory cells responding to pro-inflammatory stimuli and is considered to be an important mediator of inflammation. Both enzymes are targets for the nonsteroidal therapeutic anti-inflammatory drugs, NSAIDs. COX2 expression is significantly increased in 85-90 % of human colorectal adenocarcinomas whereas levels of COX-1 are not changed.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human COX2 protein fragment (around aa 442-572) (exact sequence is proprietary)

Isotype: IgG2b, kappa

Application Details

Application Notes:

Positive Control: Colon or Lung Carcinoma. HepG2 cells.

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (0.5-1 μg/mL)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for PTGS2

Target: PTGS2 (Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2))

Alternative Name: PTGS2

Background:

Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase, GRIPGHS, Macrophage activation-associated marker protein P71/73, PES-2, PGG/HS, PGHS-2, Prostaglandin endoperoxide synthase 2, Prostaglandin endoperoxide synthase 2 (PTGS2), TIS10

Colon or Lung Carcinoma. HepG2 cells.

Molecular Weight: 70-72kDa

Gene ID: 5743, 196384

UniProt: P35354

Pathways: Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation