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Melanoma gp100 antibody

PMEL Reactivity: Human, Horse IF, IHC, FACS, StM Host: Mouse Monoclonal SPM286 unconjugated
Catalog No. ABIN6940586
  • Target See all Melanoma gp100 (PMEL) Antibodies
    Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))
    Reactivity
    • 86
    • 11
    • 5
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Horse
    Host
    • 49
    • 35
    • 1
    Mouse
    Clonality
    • 47
    • 38
    • 1
    Monoclonal
    Conjugate
    • 45
    • 6
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Melanoma gp100 antibody is un-conjugated
    Application
    • 38
    • 36
    • 36
    • 33
    • 23
    • 14
    • 9
    • 4
    • 2
    • 2
    • 1
    Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Staining Methods (StM)
    Specificity
    By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
    No Cross-Reactivity
    Rat (Rattus)
    Purification
    Purified by Protein A/G
    Immunogen
    Membranes from a human melanoma metastasis
    Clone
    SPM286
    Isotype
    IgG2b kappa
    Top Product
    Discover our top product PMEL Primary Antibody
  • Application Notes

    Positive Control: SK-MEL-28 cells. Melanoma.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Expiry Date
    24 months
  • Target
    Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))
    Alternative Name
    SILV (PMEL Products)
    Synonyms
    D12S53E antibody, ME20 antibody, ME20-M antibody, ME20M antibody, P1 antibody, P100 antibody, PMEL17 antibody, SI antibody, SIL antibody, SILV antibody, gp100 antibody, D10H12S53E antibody, D12S53Eh antibody, Pmel17 antibody, Si antibody, Silv antibody, gp87 antibody, RPE1 antibody, MMP115 antibody, silverb antibody, silver antibody, cb397 antibody, fdv antibody, pmel17 antibody, sb:cb397 antibody, silva antibody, wu:fc11g11 antibody, wu:fj24g11 antibody, zgc:136622 antibody, premelanosome protein antibody, premelanosome protein b antibody, premelanosome protein a antibody, PMEL antibody, Pmel antibody, pmelb antibody, pmela antibody
    Molecular Weight
    90-100kDa
    Gene ID
    6490
    UniProt
    P40967
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