BRCA1 antibody (AA 445-620)

Catalog No. ABIN6940662

The Mouse Monoclonal anti-BRCA1 antibody has been validated for ELISA and Coat. It is suitable to detect BRCA1 in samples from Human.

Quick Overview for BRCA1 antibody (AA 445-620) (ABIN6940662)

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BRCA1 antibody is un-conjugated

Application: ELISA, Coating (Coat)

Clone: BRCA1-1472

Product Details anti-BRCA1 Antibody

Binding Specificity: AA 445-620

Purification: Purified by Protein A/G

Immunogen: Recombinant fragment (around aa 445-620) of human BRCA1 protein (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: A431 or HeLa cells. Breast, Ovarian or Renal Cell Carcinoma.

Known Application: ELISA (For coating use Ab at 2-4 μg/mL, order Ab without BSA), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for BRCA1

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Alternative Name: BRCA1

Background: This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40 % of inherited breast cancers and more than 80 % of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene.

Molecular Weight: 220kDa

Gene ID: 672

UniProt: P38398

Pathways: Cell Division Cycle, DNA Damage Repair, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus