VEGFA antibody

Catalog No. ABIN6940869

The Mouse Monoclonal anti-VEGFA antibody (Clone VG1) (ABIN6940869) specifically detects VEGFA in ELISA, StM, Coat and IHC (fp). The antibody is reactive with Human samples.

Quick Overview for VEGFA antibody (ABIN6940869)

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This VEGFA antibody is un-conjugated

Application: ELISA, Staining Methods (StM), Coating (Coat), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: VG1

Product Details anti-VEGFA Antibody

Specificity: This MAb recognizes proteins of 19-22 kDa (reducing) and 38 kDa-44 kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet-derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.

Purification: Purified by Protein A/G

Immunogen: Human VEGF189 recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Tumor cells in astrocytomas, breast or ovarian carcinomas.

Known Application: ELISA (For coating, order Ab without BSA),Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA buffer, pH 8.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for VEGFA

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Alternative Name: VEGFA

Molecular Weight: 19-22kDa (reducing) and 38kDa-44kDa (non-reducing)

Gene ID: 7422

UniProt: P15692

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling