Vimentin antibody

Catalog No. ABIN6940879

This Mouse Monoclonal antibody specifically detects Vimentin in WB, IHC, FACS and StM. It exhibits reactivity toward Human, Pig, Cow, Chicken, Dog, Cat and Goat.

Quick Overview for Vimentin antibody (ABIN6940879)

Target: Vimentin (VIM)

Reactivity: Human, Pig, Cow, Chicken, Dog, Cat, Goat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Staining Methods (StM)

Clone: VM452

Product Details anti-Vimentin Antibody

Specificity: This MAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

No Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Purification: Purified by Protein A/G

Immunogen: Recombinant full-length human vimentin protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Raji, Jurkat or HeLa cells. Sarcomas or Melanomas.

Known Application: Flow Cytometry (1-2 μg/million cells), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: VIM

Molecular Weight: 57-60kDa

Gene ID: 7431

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis