Cyclin B2 antibody

Catalog No. ABIN6941010

The Mouse Monoclonal anti-Cyclin B2 antibody is suitable to detect Cyclin B2 in samples from Human, Mouse, Rat and Xenopus laevis. It has been validated for ELISA, IHC and Coat.

Quick Overview for Cyclin B2 antibody (ABIN6941010)

Target: Cyclin B2 (CCNB2)

Reactivity: Human, Mouse, Rat, Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin B2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Coating (Coat)

Clone: X29-2

Product Details anti-Cyclin B2 Antibody

Purification: Purified by Protein A/G

Immunogen: Recombinant full-length Xenopus laevis Cyclin B2 protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: HeLa cells. Human cervical tissue (IHC).

Known Application: ELISA (For coating, order antibody without BSA), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Cyclin B2

Target: Cyclin B2 (CCNB2)

Alternative Name: CCNB2

Background: In eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13-Suc1). The Cdc/cyclin enzyme is subject to multiple levels of control, of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/ cyclin B enzyme, and tyrosine dephosphorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex. Evidence has established that B type cyclins not only act on M phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physiological substrate. The two B type cyclins, cyclin B1 and cyclin B2, have been shown to have distinct tissue distributions.

Molecular Weight: 51kDa

Gene ID: 9133

UniProt: O95067

Pathways: Cell Division Cycle, M Phase