CD20 antibody

Catalog No. ABIN6941110

The Mouse Monoclonal anti-CD20 antibody (Clone 93-1B3) (ABIN6941110) specifically detects CD20 in FACS, WB, IF and Func. The antibody is reactive with Human samples.

Quick Overview for CD20 antibody (ABIN6941110)

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD20 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Functional Studies (Func)

Clone: 93-1B3

Product Details anti-CD20 Antibody

Purpose: CD20 / MS4A1 (B-Cell Marker) Antibody

Specificity: Recognizes a protein of 30-33 kDa, which is identified as CD20 (Workshop V, Code CD20.4). It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. The protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B-cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as calcium channel involved in B cell activation and cell cycle progression.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Stimulated human leukocytes

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: Daudi, Raji and U266 and human lymphocytes. Lymph nodes and tonsils.

Known Application: Functional Studies (Order Ab without BSA & Azide), Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD20

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Alternative Name: MS4A1

Background:

APY, ATOPY, B-lymphocyte antigen CD20, B-lymphocyte cell-surface antigen B1, Bp35, Fc epsilon receptor I beta chain, Fc Fragment of IgE high affinity I receptor for beta polypeptide, FCER1B, High affinity immunoglobulin epsilon receptor subunit beta, IgE Fc receptor subunit beta, IGEL, IGER, IGHER, LEU16, Leukocyte surface antigen Leu-16, Ly44, Membrane spanning 4 domains subfamily A member 2, Membrane-spanning 4-domains subfamily A member 1 (MS4A1)

Daudi, Raji and U266 and human intestine, Spleen, lymphocytes. Lymph nodes and tonsils.

Molecular Weight: 33-37kDa

Gene ID: 931, 712553

UniProt: P11836