CD44 antibody

Catalog No. ABIN6941190

The Mouse Monoclonal anti-CD44 antibody (Clone 2F10) (ABIN6941190) specifically detects CD44 in FACS, WB and IF. The antibody is reactive with Human samples.

Quick Overview for CD44 antibody (ABIN6941190)

Target: CD44

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD44 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF)

Clone: 2F10

Product Details anti-CD44 Antibody

Purpose: CD44v6 (Marker of Tumor Metastasis) Antibody

Specificity: This antibody recognizes an epitope encoded by exon v6 on the variant portion of human CD44. The CD44 Molecule belongs to a family of cellular adhesion molecules found on a wide range of normal and malignant cells in epithelial, mesothelial and hemopoiesis tissues. CD44 is a single gene with 20 exons, of which 10 are normally expressed to encode the basic CD44 (H-CAM) molecule. The additional 10 exons (v1 to v10) are only expressed by alternative splicing of the nuclear RNA.The expression of specific cell adhesion molecule CD44 splice variants has been reported to be associated with metastasis in certain human malignancies.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment corresponding to the v9 domain of human CD44

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: Cervix or Tongue Squamous Cell Carcinoma.

Known Application: ELISA (For coating use Ab at 1-5 μg/mL, order Ab without BSA), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD44

Target: CD44

Alternative Name: CD44

Background:

LHR, BA-1, chondroitin sulfate proteoglycan 8 (CSPG8), Epican, Extracellular Matrix Receptor III (ECM III), GP90 Lymphocyte Homing Adhesion Receptor, HCAM, HCELL, hematopoietic cell E- and L-selectin ligand, Heparan Sulfate Proteoglycan, Hermes Antigen, HAS, HUTCH I, Hyaluronate Receptor, Indian blood group, Inlu Related p80 Glycoprotein, Ly 24, MDU2, MDU3, MIC4, MUTCH I, Phagocytic Glycoprotein 1 (PGP-1)

Cervix or Tongue Squamous Cell Carcinoma

Molecular Weight: 80-95kDa

Gene ID: 960, 502328

UniProt: P16070

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling