TNFRSF18 antibody

Catalog No. ABIN6941305

The Rat Monoclonal anti-TNFRSF18 antibody (Clone DTA-1) (ABIN6941305) specifically detects TNFRSF18 in FACS and IF. The antibody is reactive with Mouse samples.

Quick Overview for TNFRSF18 antibody (ABIN6941305)

Target: TNFRSF18 (Tumor Necrosis Factor Receptor Superfamily, Member 18 (TNFRSF18))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This TNFRSF18 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: DTA-1

Product Details anti-TNFRSF18 Antibody

Purpose: GITR / Tnfrsf18 (Mouse) Antibody

Specificity: GITR (glucocorticoid-induced TNFR-related gene) is a member of the TNF-receptor superfamily, also known as TNFRSF18. It is expressed at low levels on resting T lymphocytes and at high levels on CD25+CD4+Tregs. The expression of GITR on T cells can be upregulated upon activation. Interaction of GITR with its ligand (GITRL) has been demonstrated to augment T cell activation, proliferation, cytokine production as well as MAPKs and NF-B activation, and abrogate the inhibitory function ofCD25+CD4+Tregs. In vivoactivation of GITR causes development of autoimmune diseases and restores the suppressed immune response.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Mouse CD25+ CD4+ T cells

Isotype: IgG2b, lambda

Application Details

Application Notes:

Positive Control: CD25+CD4+Tregs.

Known Application: Flow Cytometry (0.5-1 μg/million cells in 0.1ml), Immunofluorescence (0.5-1 μg/mL), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for TNFRSF18

Target: TNFRSF18 (Tumor Necrosis Factor Receptor Superfamily, Member 18 (TNFRSF18))

Alternative Name: GITR / Tnfrsf18

Background:

Activation-inducible TNF-related ligand, AITRL, GITRL, Glucocorticoid induced TNFR related protein ligand, Glucocorticoid-induced TNF-related ligand, hGITRL, TL6, TNFSF18, Tumor necrosis factor (ligand) superfamily member 18

CD25+CD4+Tregs.

Molecular Weight: 66-70kDa (Homodimer)

Gene ID: 21936, 3180

UniProt: O35714

Pathways: Cancer Immune Checkpoints