KRT8, KRT18 antibody

Catalog No. ABIN6941355

Product Details

Target: KRT8, KRT18

Reactivity: Human, Cow, Monkey, Pig, Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)

Specificity: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells, there is very little keratin 18 in the normal epidermis so only Paget cells are stained.

No Cross-Reactivity: Chicken, Donkey, Ferret, Mouse (Murine), Rabbit, Rat (Rattus), Xenopus laevis

Purification: Purified by Protein A/G

Immunogen: Cytoskeleton preparation from HeLa cells

Clone: C-51

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: MCF-7 or A431 cells. Skin, colon, lung or breast carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details

Target: KRT8, KRT18

Alternative Name: KRT8 & KRT18

Molecular Weight: 52.5kDa (CK8), 45kDa (CK18)

Gene ID: 3856, 3875

UniProt: P05787, P05783