Recombinant MHC Class II HLA-DP/DQ/DR antibody

Catalog No. ABIN6941388

The Mouse Monoclonal anti-MHC Class II HLA-DP/DQ/DR antibody is suitable to detect MHC Class II HLA-DP/DQ/DR in samples from Human. It has been validated for FACS, IHC, WB and IF.

Quick Overview for Recombinant MHC Class II HLA-DP/DQ/DR antibody (ABIN6941388)

Target: MHC Class II HLA-DP/DQ/DR (HLA-DP/DQ/DR)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MHC Class II HLA-DP/DQ/DR antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Clone: RHLA-Pan-3475

Product Details anti-MHC Class II HLA-DP/DQ/DR Antibody

Purpose: Recombinant HLA-Pan (MHC II) Antibody

Specificity: Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an alpha chain (36 kDa) and a beta chain (27 kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six alpha and ten beta chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Non-T, non-B human acute lymphoblastic leukemia REH6 cells

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: Raji cells. Tonsil or lymph node.

Known Application: ELISA (For coating use Ab at 1-5 μg/mL, order Ab without BSA),Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL). ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for MHC Class II HLA-DP/DQ/DR

Target: MHC Class II HLA-DP/DQ/DR (HLA-DP/DQ/DR)

Alternative Name: HLA-DP, HLA-DQ, HLA-DR

Background:

HLA-DPB1, HLA-DQA1, HLA-DQB1, HLA-DRA, HLA-DRB1, HLA-DRB3, HLA-DRB4, HLA-DRB5

Daudi, Raji cells. Human tonsil or lymph node. Human Intestine, Human Lung

Molecular Weight: 33-35kDa

UniProt: P01908, P01909

Pathways: Human Leukocyte Antigen (HLA) in Adaptive Immune Response