Hepatocyte Specific Antigen antibody

Catalog No. ABIN6941433

The Mouse Monoclonal anti-Hepatocyte Specific Antigen antibody is suitable to detect Hepatocyte Specific Antigen in samples from Human and Dog. It has been validated for IF and IHC.

Quick Overview for Hepatocyte Specific Antigen antibody (ABIN6941433)

Target: Hepatocyte Specific Antigen (HepPar-1)

Reactivity: Human, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Hepatocyte Specific Antigen antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (IHC)

Clone: HepPar1

Product Details anti-Hepatocyte Specific Antigen Antibody

Purpose: HepPar-1 (Hepatocellular Marker) Antibody

Specificity: Hepatocyte Paraffin 1 or HepPar1 localizes to the mitochondria of hepatocytes. It is a sensitive marker for distinguishing hepatocellular carcinomas (HCC) from other metastatic carcinomas as well as cholangio-carcinomas. HCC's occur primarily in the stomach, but they are also found in many other organs. The Hepatocyte Specific Antigen may also be a useful marker for intestinal metaplasia. Reportedly, strong expression of the Hepatocyte Specific Antigen correlates with smaller tumor size and longer patient survival. Occasionally, Hepatocyte Specific Antigen is also found in gastric carcinomas as well as in a few other non-hepatic tumors.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Extract of a formalin-fixed, rejected-allograft of a human liver

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: Liver or Hepatocellular Carcinoma (HCC).

Known Application: Immunofluorescence (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Hepatocyte Specific Antigen

Target: Hepatocyte Specific Antigen (HepPar-1)

Alternative Name: HepPar-1 (Hepatocellular Marker)

Background:

Not Known

Liver or Hepatocellular Carcinoma (HCC).