Recombinant ARNTL antibody

Catalog No. ABIN6942287

The Rabbit Monoclonal anti-ARNTL antibody (ABIN6942287) specifically detects ARNTL in WB and FACS. The antibody is reactive with Human samples.

Quick Overview for Recombinant ARNTL antibody (ABIN6942287)

Target: ARNTL (Aryl Hydrocarbon Receptor Nuclear Translocator-Like (ARNTL))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ARNTL antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-ARNTL Antibody

Purpose: BMAL1 Recombinant Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: C terminal human BMAL1

Isotype: IgG

Application Details

Application Notes: WB(WB(1:500-2000)), FCM(FCM(1:50-100))

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M TBS ( pH 7.4), 1 % BSA, 0.02 % Proclin 300, and 50 % Glycerol

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for ARNTL

Target: ARNTL (Aryl Hydrocarbon Receptor Nuclear Translocator-Like (ARNTL))

Alternative Name: BMAL1

Background:

Synonyms: ARNTL, Aryl hydrocarbon receptor nuclear translocator-like protein 1, Basic-helix-loop-helix-PAS protein MOP3, Brain and muscle ARNT-like 1, Class E basic helix-loop-helix protein 5, Member of PAS protein 3, PAS domain-containing protein 3, bHLH-PAS protein JAP3, bHLHe5, BHLHE5, BMAL1, MOP3, PASD3.

Background: Transcriptional activator which forms a core component of the circadian clock. Transcription factors, CLOCK or NPAS2 and ARNTL/BMAL1 or ARNTL2/BMAL2, form the positive limb of the feedback loop, act in the form of a heterodimer and activate the transcription of core clock genes and clock-controlled genes (involved in key metabolic processes), harboring E-box elements (5'-CACGTG-3') within their promoters. The core clock genes: PER1/2/3 and CRY1/2 which are transcriptional repressors form the negative limb of the feedback loop and interact with the CLOCK|NPAS2-ARNTL/BMAL1|ARNTL2/BMAL2 heterodimer inhibiting its activity and thereby negatively regulating their own expression. This heterodimer also activates nuclear receptors NR1D1/2 and RORA/B/G, which form a second feedback loop and which activate and repress ARNTL/BMAL1 transcription, respectively. ARNTL/BMAL1 positively regulates myogenesis and negatively regulates adipogenesis via the transcriptional control of the genes of the canonical Wnt signaling pathway.

Gene ID: 406

UniProt: O00327

Pathways: Regulation of Lipid Metabolism by PPARalpha, Protein targeting to Nucleus, Warburg Effect