Recombinant BUB3 antibody (N-Term)

Catalog No. ABIN6942297

This Rabbit Monoclonal antibody specifically detects BUB3 in WB, FACS, IHC (p), IF (cc), IF (p) and IP. It exhibits reactivity toward Human.

Quick Overview for Recombinant BUB3 antibody (N-Term) (ABIN6942297)

Target: BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This BUB3 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunoprecipitation (IP)

Product Details anti-BUB3 Antibody

Binding Specificity: N-Term

Purpose: BUB3 Recombinant Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: Recombinant protein corresponding to the N-terminus of human Bub3.

Isotype: IgG

Application Details

Application Notes: WB(1:300-5000), FCM(1:20-100), IHC-P(1:200-400), IF(IHC-P)(1:50-200), IF(ICC)(1:50-200), IP(1-2 μg)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M TBS ( pH 7.4), 1 % BSA, 0.02 % Proclin 300, and 50 % Glycerol

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for BUB3

Target: BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))

Alternative Name: Bub3

Background:

Synonyms: Mitotic checkpoint protein BUB3, BUB3

Background: Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.

Gene ID: 9184

UniProt: O43684

Pathways: Maintenance of Protein Location