CHEK2 antibody

Catalog No. ABIN6942554

This anti-CHEK2 antibody is a Rabbit Monoclonal antibody detecting CHEK2 in WB, IHC, IF (cc) and IHC (p). Suitable for Human.

Quick Overview for CHEK2 antibody (ABIN6942554)

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CHEK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 7A4

Product Details anti-CHEK2 Antibody

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: Human Chk2 between 1-200 amino acids

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IF(ICC) 1:50-200
IHC()

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for CHEK2

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Alternative Name: Chk2

Background:

Synonyms: Serine/threonine-protein kinase Chk2, CHK2 checkpoint homolog, Cds1 homolog, Checkpoint kinase 2, Hucds1, hCds1, CHEK2

Background: In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Gene ID: 11200

UniProt: O96017

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle