Recombinant HDAC9 antibody (AA 1-150)

Catalog No. ABIN6943611

This Rabbit Monoclonal antibody specifically detects HDAC9 in WB, IHC (p), IF (cc), IF (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for Recombinant HDAC9 antibody (AA 1-150) (ABIN6943611)

Target: HDAC9 (Histone Deacetylase 9 (HDAC9))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This HDAC9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-HDAC9 Antibody

Binding Specificity: AA 1-150

Purpose: HDAC9 Recombinant Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: Recombinant protein within human HDAC9 aa 1-150

Isotype: IgG

Application Details

Application Notes: WB(WB(1:500-2000)), IHC-P(IHC-P(1:100-500)), IHC-F(IHC-F(1:100-500)), IF(IHC-P)(IF(IHC-P)(1:100-500)), IF(ICC)(IF(ICC)(1:50-200))

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M TBS ( pH 7.4), 1 % BSA, 0.02 % Proclin 300, and 50 % Glycerol

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for HDAC9

Target: HDAC9 (Histone Deacetylase 9 (HDAC9))

Alternative Name: HDAC9

Background:

Synonyms: Histone deacetylase 9, Histone deacetylase 7B, Histone deacetylase-related protein, MEF2-interacting transcription repressor MITR, HD9, HD7, HD7b, HDAC9, HDAC7, HDAC7B, HDRP, KIAA0744, MITR.

Background: In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is a critical component of transcriptional regulation and the acetylation of nucleosomal histones is a major source of this remodeling. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Several mammalian proteins function as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Conversely, the deacetylation of histones is associated with transcriptional silencing. The histone deacetylases (HDAC) include HDAC1-9. HDAC9 and HDAC9a are two alternatively spliced isoforms of HDAC9. HDAC9a is 132 amino acids shorter than HDAC9, but both isoforms contain the HDAC catalytic domain, remain capable of deacetylase activity and repress myoctye enhancer-binding factor 2-mediated transcription.

Gene ID: 9734

UniProt: Q9UKV0

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development