HYOU1 antibody

Catalog No. ABIN6943764

This Rabbit Monoclonal antibody specifically detects HYOU1 in WB, IHC (p) and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for HYOU1 antibody (ABIN6943764)

Target: HYOU1 (Hypoxia Up Regulated 1 (HYOU1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This HYOU1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: 5H4

Product Details anti-HYOU1 Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: Recombinant protein within human ORP150 aa 800-950.

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for HYOU1

Target: HYOU1 (Hypoxia Up Regulated 1 (HYOU1))

Alternative Name: HYOU1

Background:

Synonyms: Hypoxia up-regulated protein 1, 150 kDa oxygen-regulated protein, 170 kDa glucose-regulated protein, ORP-150, GRP-170, HYOU1, GRP170, ORP150

Background: ORP150, also known as HYOU1 (hypoxia upregulated 1), is a 999 amino acid protein that localizes to the lumen of the endoplasmic reticulum (ER) and is a member of the heat shock protein 70 (HSP 70) family. ORP150 is highly expressed in tissues that have well-developed ERs and a large number of secretory proteins (such as liver and pancreas). It is expressed at lower levels in kidney and brain, and plays an essential role in cytoprotective cellular responses to hypoxia (oxygen deprivation). Specifically, ORP150 participates in protein folding and secretion in the ER and functions to protect cells from hypoxia-induced apoptosis, thereby playing a crucial role in cell survival. ORP150 expression is upregulated in a variety of tumors, such as breast cancer, suggesting an important role in tumorigenesis. The gene encoding ORP150 has two translation initiation sites, resulting in a truncated transcript that lacks an ER signal peptide, but is thought to function as a housekeeping protein in the cytoplasm.

Gene ID: 10525

UniProt: Q9Y4L1

Pathways: ER-Nucleus Signaling, SARS-CoV-2 Protein Interactome