Recombinant Ki-67 antibody

Catalog No. ABIN6943883

The Rabbit Monoclonal anti-Ki-67 antibody (ABIN6943883) specifically detects Ki-67 in WB, FACS, IHC (p), IF (p), IF (cc) and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for Recombinant Ki-67 antibody (ABIN6943883)

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Ki-67 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Ki-67 Antibody

Purpose: Ki67 Recombinant Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Ki67

Isotype: IgG

Application Details

Application Notes: WB(WB(1:1000-5000)), FCM(FCM(1:50-100)), IHC-P(IHC-P(1:200-1000)), IHC-F(IHC-F(1:200-1000)), IF(IHC-P)(IF(IHC-P)(1:200-1000)), IF(ICC)(IF(ICC)(1:200-1000))

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M TBS ( pH 7.4), 1 % BSA, 0.02 % Proclin 300, and 50 % Glycerol

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for Ki-67

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Alternative Name: Ki67

Background:

Synonyms: Antigen identified by monoclonal antibody Ki 67, Antigen KI67, KIA, MKI67, Proliferation related Ki 67 antigen, Antigen KI-67, KI67_HUMAN.

Background: Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.

Gene ID: 4288

UniProt: P46013

Pathways: Glycosaminoglycan Metabolic Process