Recombinant LIG4 antibody (AA 500-700)

Catalog No. ABIN6943923

This Rabbit Monoclonal antibody specifically detects LIG4 in WB, IF, ICC, IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for Recombinant LIG4 antibody (AA 500-700) (ABIN6943923)

Target: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This LIG4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-LIG4 Antibody

Binding Specificity: AA 500-700

Purpose: DNA Ligase IV Recombinant Antibody

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: Recombinant protein within human DNA Ligase IV aa 500-700

Isotype: IgG

Application Details

Application Notes: WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF(), ICC/IF(1:50-100)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M TBS ( pH 7.4), 1 % BSA, 0.02 % Proclin 300, and 50 % Glycerol

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for LIG4

Target: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Alternative Name: LIG4

Background:

Synonyms: DNA ligase 4, DNA ligase IV, Polydeoxyribonucleotide synthase [ATP] 4, LIG4.

Background: Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.

Gene ID: 3981

UniProt: P49917

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response