NCOA1 antibody

Catalog No. ABIN6944226

Product Details anti-NCOA1 Antibody

Target: NCOA1 (Nuclear Receptor Coactivator 1 (NCOA1))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This NCOA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: Recombinant protein within human KAT13A/ SRC1 aa 450-700

Clone: 2G9

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NCOA1

Target: NCOA1 (Nuclear Receptor Coactivator 1 (NCOA1))

Alternative Name: NCOA1 (NCOA1 Products)

Synonyms: F-SRC-1 antibody, KAT13A antibody, RIP160 antibody, SRC1 antibody, bHLHe42 antibody, bHLHe74 antibody, si:dkey-5g7.6 antibody, si:dkey-34f16.6 antibody, NCOA1 antibody, SRC-1 antibody, nuclear receptor coactivator 1 antibody, NCOA1 antibody, ncoa1 antibody, Ncoa1 antibody

Background:

Synonyms: Nuclear receptor coactivator 1, Class E basic helix-loop-helix protein 74, Protein Hin-2, RIP160, Renal carcinoma antigen NY-REN-52, Steroid receptor coactivator 1, NCoA-1, bHLHe74, SRC-1, NCOA1, BHLHE74, SRC1

Background: Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4, the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via remodeling of chromatin, and possibly acts by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response. Isoform 2 has a higher thyroid hormone-dependent transactivation activity than isoform 1 and isoform 3.

Gene ID: 8648

UniProt: Q15788

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha