PKC zeta antibody (Protein Kinase C, zeta) (pThr560)

Details for Product anti-PRKCZ Antibody No. ABIN6944560
Antigen
  • TPKC
  • PKCzeta
  • prkcz-A
  • pkc-zeta
  • PRKCZ
  • pkc2
  • PKC-ZETA
  • PKC2
  • AI098070
  • C80388
  • Pkcz
  • R74924
  • aPKCzeta
  • zetaPKC
  • 14-3-3-zetaisoform
  • r14-3-3
  • pkcz
  • si:ch211-150o23.4
  • protein kinase C zeta L homeolog
  • protein kinase C zeta
  • protein kinase C, zeta
  • prkcz.L
  • PRKCZ
  • prkcz
  • Prkcz
Epitope
pThr560
50
36
24
15
9
9
7
4
4
3
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
216
159
139
11
9
8
4
4
4
2
2
2
2
2
Host
Rabbit
241
8
1
Clonality (Clone)
Monoclonal ()
Conjugate
This PKC zeta antibody is un-conjugated
12
8
7
6
6
6
6
6
6
4
4
4
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
164
109
99
38
36
18
14
13
13
5
2
2
2
2
1
1
Options
Immunogen Synthetic phospho-peptide surrounding Thr560 of human PKC zeta
Clone 26G8
Isotype IgG
Purification Purified by Protein A.
Plasmids, Primers & others Plasmids, Primers & others PKC zeta products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name PKC zeta (PRKCZ Antibody Abstract)
Background

Calcium- and diacylglycerol-independent serine/threonine-protein kinase that functions in phosphatidylinositol 3-kinase (PI3K) pathway and mitogen-activated protein (MAP) kinase cascade, and is involved in NF-kappa-B activation, mitogenic signaling, cell proliferation, cell polarity, inflammatory response and maintenance of long-term potentiation (LTP). Upon lipopolysaccharide (LPS) treatment in macrophages, or following mitogenic stimuli, functions downstream of PI3K to activate MAP2K1/MEK1-MAPK1/ERK2 signaling cascade independently of RAF1 activation. Required for insulin-dependent activation of AKT3, but may function as an adapter rather than a direct activator. Upon insulin treatment may act as a downstream effector of PI3K and contribute to the activation of translocation of the glucose transporter SLC2A4/GLUT4 and subsequent glucose transport in adipocytes. In EGF-induced cells, binds and activates MAP2K5/MEK5-MAPK7/ERK5 independently of its kinase activity and can activate JUN promoter through MEF2C. Through binding with SQSTM1/p62, functions in interleukin-1 signaling and activation of NF-kappa-B with the specific adapters RIPK1 and TRAF6. Participates in TNF-dependent transactivation of NF-kappa-B by phosphorylating and activating IKBKB kinase, which in turn leads to the degradation of NF-kappa-B inhibitors. In migrating astrocytes, forms a cytoplasmic complex with PARD6A and is recruited by CDC42 to function in the establishment of cell polarity along with the microtubule motor and dynein. In association with FEZ1, stimulates neuronal differentiation in PC12 cells. In the inflammatory response, is required for the T-helper 2 (Th2) differentiation process, including interleukin production, efficient activation of JAK1 and the subsequent phosphorylation and nuclear translocation of STAT6. May be involved in development of allergic airway inflammation (asthma), a process dependent on Th2 immune response. In the NF-kappa-B-mediated inflammatory response, can relieve SETD6-dependent repression of NF-kappa-B target genes by phosphorylating the RELA subunit at 'Ser-311'. Necessary and sufficient for LTP maintenance in hippocampal CA1 pyramidal cells. In vein endothelial cells treated with the oxidant peroxynitrite, phosphorylates STK11 leading to nuclear export of STK11, subsequent inhibition of PI3K/Akt signaling, and increased apoptosis. Phosphorylates VAMP2 in vitro (PubMed:17313651).


Synonyms: Protein kinase C zeta type, nPKC-zeta, PRKCZ, PKC2
Gene ID 5590
UniProt Q05513
Pathways NF-kappaB Signaling, RTK Signaling, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling
Application Notes WB(1:300-1000), IHC()
Restrictions For Research Use only
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Expiry Date 12 months
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