PMS2 antibody (PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae))

Details for Product anti-PMS2 Antibody No. ABIN6944621
Target
Reactivity
Human
78
13
10
2
2
2
1
1
1
1
1
1
1
1
1
Host
Rabbit
55
22
1
Clonality (Clone)
Monoclonal ()
Conjugate
This PMS2 antibody is un-conjugated
4
2
2
2
2
2
2
2
Application
Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (IHC), Western Blotting (WB)
52
34
22
17
12
8
5
3
3
2
1
1
Options
Immunogen Full length protein
Clone 9H11
Isotype IgG
Purification Purified by Protein A.
Target
Alternative Name PMS2 (PMS2 Antibody Abstract)
Background

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages.


Synonyms: Mismatch repair endonuclease PMS2, DNA mismatch repair protein PMS2, PMS1 protein homolog 2, PMS2
Gene ID 5395
UniProt P54278
Pathways DNA Damage Repair, Production of Molecular Mediator of Immune Response
Application Notes WB(1:300-1000), FCM(1:20-100), IF(ICC)(1:50-200), IHC()
Restrictions For Research Use only
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Expiry Date 12 months