PKC beta antibody

Catalog No. ABIN6944656

The Rabbit Monoclonal anti-PKC beta antibody has been validated for WB, IHC, FACS, IF (cc) and IHC (p). It is suitable to detect PKC beta in samples from Human, Rat and Mouse.

Quick Overview for PKC beta antibody (ABIN6944656)

Target: PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PKC beta antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 10A1

Product Details anti-PKC beta Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: Human PKC beta 2 between 600 to C-terminus

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(ICC) 1:50-200
IHC()

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for PKC beta

Target: PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))

Alternative Name: PRKCB

Background:

Synonyms: Protein kinase C beta type, PKC-B, PKC-beta, PRKCB, PKCB, PRKCB1

Background: Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional PKC isoforms (_, _I, _II and _) and novel PKC isoforms. Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC _ and are independent of Ca2+. On the other hand, nPKC _ and _, as well as all of the cPKC members, possess phorbol ester-binding activities and kinase activities.

Gene ID: 5579

UniProt: P05771

Pathways: WNT Signaling, TCR Signaling, Thyroid Hormone Synthesis, Nuclear Hormone Receptor Binding, Chromatin Binding, Myometrial Relaxation and Contraction, VEGF Signaling, Unfolded Protein Response, BCR Signaling