PTBP1 antibody

Catalog No. ABIN6944694

This Rabbit Monoclonal antibody specifically detects PTBP1 in WB and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PTBP1 antibody (ABIN6944694)

Target: PTBP1 (Polypyrimidine Tract Binding Protein 1 (PTBP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PTBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 7D3

Product Details anti-PTBP1 Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: Recombinant protein within human PTBP1 1-200

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PTBP1

Target: PTBP1 (Polypyrimidine Tract Binding Protein 1 (PTBP1))

Alternative Name: PTBP1

Background:

Synonyms: Polypyrimidine tract-binding protein 1, 57 kDa RNA-binding protein PPTB-1, Heterogeneous nuclear ribonucleoprotein I, PTB, hnRNP I, PTBP1.

Background: PTBP1 belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). PTBP1 a role in pre-mRNA splicing and in the regulation of alternative splicing events. Activates exon skipping of its own pre-mRNA during muscle cell differentiation. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. Represses the splicing of MAPT/Tau exon 10. In case of infection by picornaviruses, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation.

Gene ID: 5725

UniProt: P26599

Pathways: Regulation of Muscle Cell Differentiation