Sonic Hedgehog antibody (AA 250-350)

Catalog No. ABIN6944987

The Rabbit Monoclonal anti-Sonic Hedgehog antibody (Clone 4C10) (ABIN6944987) specifically detects Sonic Hedgehog in WB, IHC, FACS, IHC (p) and IF (cc). The antibody is reactive with Human samples.

Quick Overview for Sonic Hedgehog antibody (AA 250-350) (ABIN6944987)

Target: Sonic Hedgehog (SHH)

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Sonic Hedgehog antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Clone: 4C10

Product Details anti-Sonic Hedgehog Antibody

Binding Specificity: AA 250-350

Purpose: Sonic Hedgehog Protein (C-Product) (4C10) Monoclonal Antibody

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: Human Sonic Hedgehog between 250-350 amino acids

Isotype: IgG

Application Details

Application Notes: WB(1:300-5000), FCM(1:200-400), IHC-P(1:100-500), IF(ICC)(1:50-200), IHC()

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for Sonic Hedgehog

Target: Sonic Hedgehog (SHH)

Alternative Name: Sonic Hedgehog Protein (C-Product)

Background:

Synonyms: Sonic hedgehog protein, HHG-1, SHH

Background: Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO (By similarity).

Gene ID: 6469

UniProt: Q15465

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development