HIST1H4A antibody (acLys16)

Catalog No. ABIN6946913

Product Details anti-HIST1H4A Antibody

Target: HIST1H4A (Histone Cluster 1, H4a (HIST1H4A))

Binding Specificity: acLys16

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This HIST1H4A antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: Synthetic peptide corresponding to human histone H4 (acetyl K16).

Clone: 2G2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for HIST1H4A

Target: HIST1H4A (Histone Cluster 1, H4a (HIST1H4A))

Alternative Name: HIST1H4A (HIST1H4A Products)

Synonyms: Hist1h4a antibody, H4FA antibody, histone cluster 1, H3f antibody, histone cluster 1 H4 family member a antibody, histone cluster 1, H4a antibody, Hist1h3f antibody, HIST1H4A antibody, Hist1h4a antibody

Background:

Synonyms: Histone H4, HIST1H4A, acetyl-Histone H4 (Lys16), H4K16Ac, histone cluster 4 H4, Histone H4 Acetylated on Lysine 16, H4 histone family

Background: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H4 are known to be acetylated on Lysine 5, 8, 12, and 16. Acetylations are important for the regulation of histone deposition, transcriptional activation, DNA replication and repair. Hyper-acetylation of the histone tails is shown to neutralize positive charge and weakens histone-DNA and nucleosome-nucleosome interactions that destabilizes chromatin structure and increases the accessibility of DNA to various DNA-binding proteins.

Gene ID: 121504

UniProt: P62805