POLR2A/RPB1 antibody (pSer5)

Catalog No. ABIN6952400

This Mouse Monoclonal antibody specifically detects POLR2A/RPB1 in WB, IF, ELISA, ChIP and ChIP-seq. It exhibits reactivity toward Human.

Quick Overview for POLR2A/RPB1 antibody (pSer5) (ABIN6952400)

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This POLR2A/RPB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

Grade: ChIP-seq Grade

Product Details anti-POLR2A/RPB1 Antibody

Binding Specificity: pSer5

Specificity: Monoclonal antibody raised in mouse against the YSPTSPS repeat in the B1 subunit of RNA polymerase II, phosphorylated at Ser5 of the repeat sequence.

Purification: Protein A purified

Isotype: IgG1

Application Details

Application Notes: ChIP/ChIP-seq 1 - 2 μg:ChIP
ELISA 1:3,000
WB 1:1,000
IF 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: PBS, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for POLR2A/RPB1

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Alternative Name: Pol II

Background: RNA polymerase II (pol II) is a key enzyme in the regulation and control of gene transcription. It is able to unwind the DNA double helix, synthesize RNA, and proofread the result. Pol II is a complex enzyme, consisting of 12 subunits, of which the B1 subunit (UniProt/Swiss-Prot entry P24928) is the largest. Together with the second largest subunit, B1 forms the catalytic core of the RNA polymerase II transcription machinery.

UniProt: P24928

Pathways: Regulatory RNA Pathways