POLR2A/RPB1 antibody

Catalog No. ABIN6952401

The Mouse Monoclonal anti-POLR2A/RPB1 antibody is suitable to detect POLR2A/RPB1 in samples from Human. It has been validated for WB, ChIP and ChIP-seq.

Quick Overview for POLR2A/RPB1 antibody (ABIN6952401)

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This POLR2A/RPB1 antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

Grade: ChIP-seq Grade

Product Details anti-POLR2A/RPB1 Antibody

Specificity: Monoclonal antibody raised in mouse against the B1 subunit of RNA polymerase II (polymerase (RNA) II (DNA directed) polypeptide A) of wheat germ. Interacts with the highly conserved C-terminal domain of the protein containing the YSPTSPS repeat.

Cross-Reactivity: Mouse (Murine), Xenopus laevis

Purification: ascites fluid

Isotype: IgG2a

Application Details

Application Notes: ChIP/ChIP-seq 2 μL:ChIP
WB 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: ascites fluid, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for POLR2A/RPB1

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Alternative Name: Pol II

Background: RNA polymerase II (pol II) is a key enzyme in the regulation and control of gene transcription. It is able to unwind the DNA double helix, synthesize RNA, and proofread the result. Pol II is a complex enzyme, consisting of 12 subunits, of which the B1 subunit (UniProt/Swiss-Prot entry P24928) is the largest. Together with the second largest subunit, B1 forms the catalytic core of the RNA polymerase II transcription machinery.

UniProt: P24928

Pathways: Regulatory RNA Pathways