CD80 antibody (APC)

Catalog No. ABIN7278001

This Armenian Hamster Monoclonal antibody specifically detects CD80 in FACS. It exhibits reactivity toward Mouse.

Quick Overview for CD80 antibody (APC) (ABIN7278001)

Target: CD80

Reactivity: Mouse

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This CD80 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Clone: 16-10A1

Product Details anti-CD80 Antibody (APC)

Purification: This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.

Isotype: IgG

Application Details

Application Notes: This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indicated). Please refer to the figure legend for the optimal concentration used to stain the tissue shown. We recommend titrating the antibody under your specific conditions to determine the optimal concentration of antibody needed in your experimental system.

Comment:

0.2 mg/mL

Restrictions: For Research Use only

Handling

Buffer: 10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, 0.1 % gelatin, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C protected from light

Expiry Date: 12 months

Target Details for CD80

Target: CD80

Alternative Name: CD80 (B7-1)

Background: The 16-10A1 antibody reacts with mouse CD80, also known as B7-1, a 55 kDa type I transmembrane protein ligand for CD152 (CTLA-4) and for CD28, a co- stimulatory receptor for the T cell receptor (TCR). CD28 also binds a second B7 ligand known as CD86 (B7-2). Both CD80 and CD86 are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 or CTLA-4 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response.

Gene ID: 12519

UniProt: Q00609

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints