Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

IL-4 antibody (PromoFluor 647 Premium)

IL4 Reactivity: Human, Non-Human Primate FACS Host: Rat Monoclonal IL4-3 PromoFluor 647 Premium
Catalog No. ABIN6963828
  • Target See all IL-4 (IL4) Antibodies
    IL-4 (IL4) (Interleukin 4 (IL4))
    Reactivity
    • 135
    • 57
    • 22
    • 14
    • 11
    • 10
    • 10
    • 8
    • 7
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    Human, Non-Human Primate
    Host
    • 110
    • 59
    • 55
    • 7
    • 3
    • 2
    Rat
    Clonality
    • 126
    • 107
    • 1
    Monoclonal
    Conjugate
    • 139
    • 33
    • 16
    • 13
    • 10
    • 8
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    This IL-4 antibody is conjugated to PromoFluor 647 Premium
    Application
    • 119
    • 110
    • 70
    • 60
    • 25
    • 20
    • 19
    • 16
    • 13
    • 13
    • 12
    • 11
    • 9
    • 9
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Specificity
    Native IL-4
    Cross-Reactivity (Details)
    The monoclonal antibody cross-reacts with IL-4 from non-human primates.
    Purification
    Purified from in vitro cultures by protein G affinity chromatography.
    Immunogen
    Recombinant human IL-4
    Clone
    IL4-3
    Isotype
    IgG1
    Top Product
    Discover our top product IL4 Primary Antibody
  • Application Notes
    Detection of IL-4 producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. Detection of IL-4 by flow cytometry in viable human PBMC. Cells were stimulated for 16 hours in the presence of PMA/ionomycin and Brefeldin A. Cells were stained with anti-CD3-BV421, fixed and permeabilized using 4 % paraformaldehyde and saponin, and further stained with PF647P-conjugated mAb IL4-3 (right panel). Non-stimulated cells shown as reference (left panel). The dot plot derives from gated events of typical lymphocyte characteristics in forward and side light scatter. Flow cytometry was performed on a BD FACSVerse system.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    in PBS with 0.2 % BSA and 0.09 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
    Expiry Date
    18 months
  • Target
    IL-4 (IL4) (Interleukin 4 (IL4))
    Alternative Name
    IL4 (IL4 Products)
    Synonyms
    IL4 antibody, IL-4 antibody, BCGF-1 antibody, BCGF1 antibody, BSF-1 antibody, BSF1 antibody, Il-4 antibody, Il4e12 antibody, IL2 antibody, interleukin 4 antibody, IL4 antibody, Il4 antibody
    Gene ID
    3565
    Pathways
    JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation
You are here:
Support