IL-2 antibody

Catalog No. ABIN6963857

This Mouse Monoclonal antibody specifically detects IL-2 in ELISA and ELISpot. It exhibits reactivity toward Human and Non-Human Primate and has been mentioned in 4+ publications.

Quick Overview for IL-2 antibody (ABIN6963857)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-2 antibody is un-conjugated

Application: ELISA, ELISpot

Clone: MT2A91-2C95

Product Details anti-IL-2 Antibody

Specificity: Native and recombinant IL-2

Cross-Reactivity (Details): The monoclonal antibodies cross-react with IL-2 from non-human primates.

Characteristics: These monoclonal antibodies enable specific detection of human IL-2 in immunoassays such as ELISpot, FluoroSpot, and ELISA. Serum/Plasma samples

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Sterility: 0.2 μm filtered

Immunogen: Recombinant human IL-2

Isotype: IgG1, IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

ABIN6963855 and ABIN6963856/2C95 are recommended as capture mAbs in ELISpot, FluoroSpot, and ELISA in combination with detection mAb ABIN6963858, ABIN7448302 and ABIN6963859 .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: supplied at 0.5 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store product at 4-8°C.

Expiry Date: 18 months

References for anti-IL-2 antibody (ABIN6963857)

Starc, Li, Algeri, Conforti, Tomao, Pitisci, Emma, Montini, Messa, Locatelli, Bernardo, Vivarelli: "Phenotypic and functional characterization of mesenchymal stromal cells isolated from pediatric patients with severe idiopathic nephrotic syndrome." in: Cytotherapy, Vol. 20, Issue 3, pp. 322-334, (2019) (PubMed).

Ashrafi, Shapouri, Mahdavi: "Immunological consequences of immunization with tumor lysate vaccine and propranolol as an adjuvant: A study on cytokine profiles in breast tumor microenvironment." in: Immunology letters, Vol. 181, pp. 63-70, (2017) (PubMed).

Douthwaite, Moisan, Privezentzev, Soskic, Sabbah, Cohen, Collinson, England, Huntington, Kemp, Zhuang, Hudak, Rees, Goldberg, Barton, Chang, Vainshtein, Liang, Iciek, Ambery, Peakman, Vaughan, Tree et al.: "A CD80-Biased CTLA4-Ig Fusion Protein with Superior In Vivo Efficacy by Simultaneous Engineering of Affinity, Selectivity, Stability, and FcRn Binding. ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 198, Issue 1, pp. 528-537, (2017) (PubMed).

Forghieri, Luppi, Barozzi, Riva, Morselli, Bigliardi, Quadrelli, Vallerini, Maccaferri, Coluccio, Paolini, Colaci, Bonacorsi, Maiorana, Tagliazucchi, Rumpianesi, Mattioli, Presutti, Gelmini, Cermelli et al.: "Chronic and recurrent benign lymphadenopathy without constitutional symptoms associated with human herpesvirus-6B reactivation. ..." in: British journal of haematology, Vol. 172, Issue 4, pp. 561-72, (2016) (PubMed).

Target Details for IL-2

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: IL2

Gene ID: 3558

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation