IL-2 antibody (Biotin)

Catalog No. ABIN6963858

The Mouse Monoclonal anti-IL-2 antibody has been validated for ELISA, FACS and ELISpot. It is suitable to detect IL-2 in samples from Human and Non-Human Primate. There are 4+ publications available.

Quick Overview for IL-2 antibody (Biotin) (ABIN6963858)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-2 antibody is conjugated to Biotin

Application: ELISA, Flow Cytometry (FACS), ELISpot

Clone: MT8G10

Product Details anti-IL-2 Antibody (Biotin)

Specificity: Native and recombinant IL-2

Cross-Reactivity (Details): The monoclonal antibody cross-reacts with IL-2 from non-human primates.

Characteristics: This monoclonal antibody enables specific detection of human IL-2 in immunoassays such as ELISpot, FluoroSpot, ELISA, and flow cytometry. Serum/Plasma samples

Purification: Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.,Purified from in vitro cultures by protein G affinity chromatography.

Sterility: 0.2 μm filtered

Immunogen: Recombinant human IL-2

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

ABIN6963858, ABIN7448302 and ABIN6963859 is recommended as detection mAb in ELISpot, FluoroSpot, and ELISA in combination with capture mAbs ABIN6963855 and ABIN6963856/2C95 . ABIN6963858, ABIN7448302 and ABIN6963859 is also recommended for use in flow cytometry.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: supplied at 0.5 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-IL-2 antibody (Biotin) (ABIN6963858)

Song, Zheng, Liu, Tian, Wu, Ma, Chen, Zou, Wang, Wang, Zhang, Lu, Wu, Wu: "Deficient humoral responses and disrupted B-cell immunity are associated with fatal SFTSV infection." in: Nature communications, Vol. 9, Issue 1, pp. 3328, (2018) (PubMed).

Mazor, Kaplan, Park, Jang, Lee, Kreitman, Pastan: "Rational design of low immunogenic anti CD25 recombinant immunotoxin for T cell malignancies by elimination of T cell epitopes in PE38." in: Cellular immunology, Vol. 313, pp. 59-66, (2017) (PubMed).

Ashrafi, Shapouri, Mahdavi: "Immunological consequences of immunization with tumor lysate vaccine and propranolol as an adjuvant: A study on cytokine profiles in breast tumor microenvironment." in: Immunology letters, Vol. 181, pp. 63-70, (2017) (PubMed).

Douthwaite, Moisan, Privezentzev, Soskic, Sabbah, Cohen, Collinson, England, Huntington, Kemp, Zhuang, Hudak, Rees, Goldberg, Barton, Chang, Vainshtein, Liang, Iciek, Ambery, Peakman, Vaughan, Tree et al.: "A CD80-Biased CTLA4-Ig Fusion Protein with Superior In Vivo Efficacy by Simultaneous Engineering of Affinity, Selectivity, Stability, and FcRn Binding. ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 198, Issue 1, pp. 528-537, (2017) (PubMed).

Target Details for IL-2

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: IL2

Gene ID: 3558

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation